BREAKTHROUGH
DISCOVERY
Tet-assisted bisulfite sequencing (TAB-Seq), a new method for mapping methylation to single base resolution of entire genome
DNA methylation regulating gene expression is a known fact but study of this 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of a method to map it at single-base resolution on a genome-wide scale. Affinity purification-based methods (which are used currently) cannot precisely locate 5hmC nor accurately determine its relative abundance at each modified site. For the first time a revolutionary genome-wide approach named “ Tet-assisted bisulfite sequencing (TAB-Seq)” has been devised by Scientists from University of Chicago, Ludwig Institute for Cancer Research, University of California, San Diego and Emory University. This method, when combined with traditional bisulfite sequencing can be used for
