A new method called Transfer PCR (TPCR) which combines PCR amplification of a gene present in a vector / plasmid and subsequent integration of the PCR product into the recipient vector without intermediate product purification and without the need of any commercial kit has been discovered by Researchers from Hebrew University of Jerusalem,and Weizmann Institute of Science, Israel.
For DNA cloning, the donor and the recipient plasmids are present in the same reaction tube together with the other reaction components, including primers, reaction buffer and dNTPs. The two primers include sequences, which are complimentary to the target gene at the 3′ end and sequences corresponding to the integration site in the recipient vector at the 5′ end.
{Image courtesy: Journal of Structural Biology)
Example:
The transcription factor IIE α subunit (ΤFIIEα, ∼1300 bp) gene is in
